Monday -Design lab

Today we reviewed your pineapple enzyme design labs and chose 2 possible ideas.

1. Independent Variable - juice type (orange, peach, grape, lemon).


2. Independent Variable - temperature (85, 60, 37, 24)

Part of your homework again tonight is to write up the PROCEDURE. You must have it written out when you arrive tomorrow.

Remember to use your original lab as a guide. You need to have a list of all the materials you need and then the steps (i.e. how to make the jello, how much to add to each tube, how many tubes does each group need). AND then a list of the IV, DEP, etc.

example from your original lab

1.      Label test tubes as “pineapple – hot,” “pineapple – RT,” “apple juice – hot,” “apple juice – RT,” and “water.”  Apply the tape at the top of the test tube with a “tail” as shown at right, so steam from the water bath does not remove the labels.

2.      Prepare gelatin in an appropriate beaker.

a.    Dissolve the contents of a gelatin packet in ¼ cup cold water.

b.    Add ¾ cup boiling water to the mixture and stir until completely dissolved.

3.      Pipette 3ml of juice or water into each test tube according to the labels, using transfer pipettes.  Do not cross-contaminate juices by using a pipette in different types of juice.

4.      Place your “hot” tubes in a beaker of water.  Heat the beaker on the hotplate until a thermometer in the water of the beaker reads 85°C.  Carefully remove the tubes from the heat.  Allow contents to come to room temperature (RT).

5.      Add 10 ml ROOM TEMPERATURE gelatin mixture to each test tube.  Use the big pipette for this and do not let the gelatin drip into the green pipetter.  Shake tubes well to ensure mixing of contents

6.      Refrigerate test tubes overnight.

We also reviewed the sickle cell gel electrophoresis handout. If asked could you answer the following questions..
1. What is the phenotype if someone is Aa? Explain
2. What is a recessive allele?
3. How do we use restriction enzymes to determine genotype of sickle cell?

The last part of your homework is the handout below

7 text homework 5 from lvilleDrFox

More on Restriction Enzymes and Gel Electrophoresis

Today we reviewed Restriction Enzymes and Gel Electrophoresis.

Homework for tomorrow: handout from today and prelab questions

Here is a great site for more practice with gel prediction, plasmids, and restriction enzymes.
Practice Reading a Gel (you can do the entire game for more instruction).

Here is a video that also takes you through a tutorial of what we did today.

Restriction Enzymes

Today we discussed Restriction Enzymes and Gel Electrophoresis.

Watch the video from start to 9:28 and then using the notes from today and the video:
 Can you answer the following questions?
1. In what organism do you find restriction enzymes?
2. What are restriction enzymes used for in that organism?
3. What are the nucleotide sequences that a restriction enzyme looks for called?
4. Why does the organism's own DNA not get cut into pieces?
5. What are the small circular pieces of DNA called in the organism and what is usually found on these circles?


We also went over how to tell band size resulting from plasmids being cut by restriction enzymes.

Here is a video (watch till 4:50) on how to read a gel


 We also began talking about Gel Electrophoresis. (remember this is also in the above video)

Here is the virtual lab link
Can you answer the questions below?
1. What does GE do?
2. What type of charge does DNA have?
3. What do the black and red cords represent?
4. Which DNA 'runs' closer to the bottom (small or large)?

Here is the picture you will use from your lab to answer the postlab questions (the wells are on the left)