Design lab - Enzymes and how they work

On Tuesday you designed your own lab to test if different fruit juices had proteolytic enzymes. Your reasoning was that like pineapple juice, orange, grapefruit, and lemon were all acidic and that could correlate with the presence of the enzyme.

Below is your Protocol
Materials
Pineapple HT/RT, Orange HT/RT, Grapefruit HT/RT, Lemon (pasteurized) HT/RT.
9 Test tubes
Hotplate, beaker with water
Gelatin (source of the collagen)
Kettle to heat water
Thermometer
Clamp
Water
Tape, Marker

Methods
1. Label test tubes (Pineapple HT/RT, Orange HT/RT, Grapefruit HT/RT, Lemon (pasteurized) HT/RT, water)
2.  Prepare Gelatin, mix in 1 packet of gelatin with 1/4 cold water and stir to somewhat dissolved. Then add in 3/4 cup of hot/boiling water and stir till all dissolved. Let cool to RT.
3. Turn on hot plate to high
3. Pipette 3 mL of each juice into appropriate tubes
4. Place the tubes labeled HT into the beaker on the hot plate. When the temp reaches 85C remove the tubes and cool them to RT
5. Add 10 mL of gelatin to each tube (both RT and HT)
6. Place tubes in rack then in fridge
7. Clean up your bench space!


Results!
Below are your results. Can you answer the following questions?

Pineapple design results from lvilleDrFox


1. What claim can you make based on the results and the results from your previous lab?
2. What is your evidence for this claim?
3. How valid is your research? How can you make it better?
4. If you wanted to redesign the experiment, what would you change?